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Structure group

Koh Takeuchi, Ph.D.

Department of Biophysical Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo


Our Techniques


Takeuchi Laboratory is developing a method to describe and understand how a life dynamically functions in a physiological environment, using atomic-resolution structure and dynamics information of biomolecules obtained from nuclear magnetic resonance (NMR) technology. We are also trying to establish a strategy to prove the biological functions of key biomolecules by controlling their activities using biochemical and biophysical techniques.
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GTP Project


Since GTP is a vital energy molecule comparable to ATP, its distribution and concentration in living cells are dynamically regulated. Our laboratory contributed to the experimental proof that the inositol phospholipid kinase, PI5P4Kβ, is an intracellular GTP sensors by constructing a single-point mutant that only lacks its GTP-dependent activity using the information derived from NMR technology. In addition, by creating a specific inhibitor of PI5P4Kβ, we have also shown that cancer actively uses the GTP sensor, PI5P4Kβ, for its growth. The study of GTP represents a fundamental principle of how living organisms can survive in an ever-changing environment by expanding the functions of proteins and other components as they evolve and monitoring their own and surrounding conditions on time while consuming or producing what they need. Thus, GTP research is the one that cuts into the basic principles of life.
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For young researchers


Takeuchi Lab will develop strategies to quantitatively understand the principles that biomolecules dynamically exert their function by combining not only NMR that can extract various structural and dynamics information to bridge structure and function, but also various molecular biological, biochemical, and biophysical techniques such as site-specific mutagenesis, isothermal titration calorimetry (ITC), surface plasmon resonance (SPR), and diffusion kinetic experiments. We are also working hard to establish platform technologies for structure-based drug discovery techniques for various modalities, which take account of their dynamics in various physiological conditions. We are always welcome motivated students and postdocs. Please feel free to contact us and join us to cut into the principle of life.

H30-R1 University of Tsukuba Tsukuba Clinical Medical Research and Development Organization "Translational Research Strategic Promotion Program"


Finished grant
 

Construction of a new anticancer drug for p53 mutant cancer targeting the GTP detection mechanism

GTP GEEKS is engaged in GTP metabolism research using various approaches such as biochemical and structural methods. Each lab accepts master's and doctoral students (and postdocs).
If you are interested in GTP study, those who are considering go to the master's doctoral feel free to Contact Please contact us from.

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