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Atsushi Kawaguchi, Ph.D.
Professor, Department of Infection Biology, Institute of Medicine, University of Tsukuba
Our Techniques
In the Kawaguchi laboratory, we are advancing research with an integrated approach that spans multiple levels, from molecular to organ and individual levels, to uncover the molecular basis of infectious diseases. We employ cutting-edge optical microscopy for cell biological analyses, work with highly pathogenic strains in BSL3 facilities, establish infection animal models using gene editing techniques, and analyze clinical samples in collaboration with the University of Tsukuba Hospital. Through this multifaceted approach, we are driving forward infectious disease research.

GTP Project
A part of GTP-binding proteins induced or functionally regulated in response to viral infection, such as MxA, GBP1, and Drp1, exhibit relatively weak GTP-binding affinity, with dissociation constants (Ka) in the range of 10–100 μM. This contrasts with typical GTPase with much higher GTP-binding affinity (Ka = 10–100 nM). Moreover, most infection-associated GTPases are activated exclusively by GTP, yet they also retain weak binding affinity for ATP. These characteristics suggest that the activity of such GTPases is fine-tuned not only by expression levels but also by local changes in GTP concentration to minimize excessive inflammation or autoimmune damage. Our group previously demonstrated that MxA, an interferon-inducible dynamin-like GTPase, functions as a pathogen sensor in a GTP-dependent manner during the host defense against influenza virus infection (Science Immunology, 2019). We aim to quantitatively analyze the spatiotemporal dynamics of intracellular GTP during viral infection and elucidate the fine-tuning mechanisms of infection-responsive GTP-binding proteins.

For young researchers
At the Department of Infection Biology, Institute of Medicine, University of Tsukuba, we focus on the infection mechanisms of respiratory viruses, including influenza viruses and SARS-CoV-2, as well as the molecular basis of host responses to these pathogens. By integrating biochemical and cell biological approaches with infection models using genetically engineered mice and structural biology techniques, we comprehensively investigate the dynamic interactions between viruses and host systems. We welcome motivated students and postdoctoral researchers who are interested in the molecular mechanisms of infectious diseases, inflammation & immunity. Laboratory visits are always welcome, please feel free to contact us at any time.
GTP GEEKS is engaged in GTP metabolism research using various approaches such as biochemical and structural methods. Each lab accepts master's and doctoral students (and postdocs).
If you are interested in GTP study, those who are considering go to the master's doctoral feel free to Contact Please contact us from.
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